KMID : 0606920160240040418
|
|
Biomolecules & Therapeutics 2016 Volume.24 No. 4 p.418 ~ p.425
|
|
Antinociceptive Effects of Prim-O-Glucosylcimifugin in Inflammatory Nociception via Reducing Spinal COX-2
|
|
Wu Liu Qing
Li Yu Li Yuan Yan Xu Shi hao Yang Zong Yong Lin Zheng Li Jun
|
|
Abstract
|
|
|
We measured anti-nociceptive activity of prim-o-glucosylcimifugin (POG), a molecule from Saposhnikovia divaricate (Turcz) Schischk. Anti-nociceptive or anti-inflammatory effects of POG on a formalin-induced tonic nociceptive response and a complete Freund¡¯s adjuvant (CFA) inoculation-induced rat arthritis pain model were studied. Single subcutaneous injections of POG produced potent anti-nociception in both models that was comparable to indomethacin analgesia. Anti-nociceptive activity of POG was dose-dependent, maximally reducing pain 56.6% with an ED50 of 1.6 mg. Rats given POG over time did not develop tolerance. POG also time-dependently reduced serum TNF¥á, IL-1¥â and IL-6 in arthritic rats and both POG and indomethacin reduced spinal prostaglandin E2 (PGE2). Like indomethacin which inhibits cyclooxygenase-2 (COX-2) activity, POG dose-dependently decreased spinal COX-2 content in arthritic rats. Additionally, POG, and its metabolite cimifugin, downregulated COX-2 expression in vitro. Thus, POG produced potent anti-nociception by downregulating spinal COX-2 expression.
|
|
KEYWORD
|
|
Prim-o-glucosylcimifugin, Nociception, Inflammation, Cytokine, Prostaglandin E2, Cyclooxygenase-2
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|